The IACUC instructs all researchers who conduct survival surgical procedures or who assist with survival surgery (such as monitoring anesthesia and monitoring post-op) to have a PASSR once approximately every 3 y. In order to ensure compliance with regulatory requirements and optimal animal welfare, our Institutional Animal Care and Use Program has developed a unique and innovative aspect of the postapproval monitoring program, the postapproval survival surgery review (PASSR). Institutional Animal Care & Use Program, University of California San Francisco, San Francisco, CA P2 The Postapproval Survival Surgery Review: A Postapproval Monitoring Tool to Evaluate Compliance and Improve Animal Welfare It is, therefore, a major refinement in welfare terms by reducing the need for invasive implants or injection regimes and by reducing side effects, while still generating consistent scientific outcomes.
Administration via diet provides a simple solution for oral drug delivery and is easily transferable to a broad range of other animal model systems requiring regular oral dosing of substances such as stimulation of transgenic models.
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To this end, we have recently been involved in a therapy trial whereby a novel test compound for oral delivery was formulated as a diet. Also, oral dosing can be stressful with welfare implications for the animal, requiring frequent restraint and invasive procedures.
This led us to consider whether oral therapeutic drugs could also be delivered via diet, since ensuring the required dose is achieved via drinking water is difficult, due to spillage or reluctance to drink water containing unpleasant tasting compounds. A test using the MCF-7 breast tumor cell line demonstrated that growth, while slower than in the pellet bearing mice, was sufficient for our studies, with no side effects apart from slight urinary retention, which was eliminated by removing the fortified diet for a few days. After this success, we decided to adopt this same method for 17-β-Estradiol delivery, because these pellets can also cause additional side effects such as bladder calculi and urine scald, which we hoped would be alleviated by changing the delivery method.
Then we tested it using the LNCaP prostate tumor cell line and found it to be as effective in stimulating growth as the pellets. We worked with a commercial diet company to formulate and produce the diet to ensure correct dosage delivery. We decided the best method for both staff and animals would be to supply it in a fortified diet, which would be less invasive and stressful to the animals while being simpler for the staff to deliver. However, due to supply issues with the 5-α-DHT pellets, we had to develop another way to provide this hormone. Traditionally, the supplement was delivered by subcutaneous slow-release pellets. P1 Food for Thought: The Development of Drug-loaded Diets to Improve Both Science and WelfareĪA Ritchie *, P Clarke, P Collier, A GrabowskaĬancer Biology, University of Nottingham, Nottingham, United KingdomĪs an in vivo cancer unit, we grow human tumors in immunodeficient mice such as CD-1 nude, which may require the addition of hormones to promote the growth of prostate and breast tumors.
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